A Simplified Method for the Determination of Total Iron-Binding Capacity of the Serum (TIBC)

Simplified determination of serum iron and total iron-binding capacity.

2,4,6-Tripyridyl-s-triazine (TPTZ) is a color reagent for ferrous ion in the pH range 2 to 10,when ascorbic acid is used as the reducing agent. In this range TPTZ and ferrous ion combine in the ratio of two to one. Tris buffer is commonly used to maintain an alkaline pH, but it reacts with other reducing agents, rendering them ineffective. These observations suggested a method for the joint det...

Modification of fully automated total iron-binding capacity (TIBC) assay in serum and comparison with dimension TIBC method.

BACKGROUND We previously reported the development of a fully automated assay for total iron-binding capacity (TIBC) in serum, using a multipurpose automated analyzer. However, this method requires four different reagents and is thus useful only with a limited number of available analyzers. We simplified our original assay and compared the analytical performance of the modified method with that ...

Diagnostic Accuracy of Serum Iron and Total Iron Binding Capacity (TIBC) in Iron Deficiency State.

OBJECTIVE To determine the diagnostic accuracy of serum iron and total iron binding capacity (TIBC) in detection of iron deficiency. STUDY DESIGN Descriptive, analytical study. PLACE AND DURATION OF STUDY Department of Chemical Pathology and Endocrinology, from January 2013 to October 2015. METHODOLOGY Data of 1,815 patients with results of serum iron, TIBC and ferritin from January 2013 ...

New method for determining total iron-binding capacity of serum (TIBC) with radioiron by eliminating iron from transferrin.

Determination of the Serum Iron-binding Capacity.

A colorimetric and an isotopic method for determining the iron-binding capacity are described, using only 1 ml. samples of plasma or serum. Both methods are simple, rapid and sensitive. Satisfactory results are obtained from frozen as well as from fresh samples.